| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Madison M (15)
|
NIVICT |
4:51.31 |
5:03.30 |
11.99 |
| 2 |
Senglaub, Katie (14)
|
NIVICT |
4:58.76 |
5:04.16 |
5.40 |
| 3 |
McKane, Katherine V (16)
|
NIHAWK |
5:19.92 |
5:18.85 |
-1.07 |
| 4 |
Romanick, Paige K (13)
|
NIPACK |
6:07.60 |
5:22.96 |
-44.64 |
| 5 |
Jackel, Summer N (16)
|
NIGRSC |
5:20.09 |
5:23.51 |
3.42 |
| 6 |
Werges, Lillian F (13)
|
NIVICT |
|
5:25.26 |
- |
| 7 |
Turner, Vanessa L (13)
|
NIHAWK |
5:27.15 |
5:29.12 |
1.97 |
| 8 |
Salonen, Amber (15)
|
NIGCOM |
5:12.51 |
5:32.53 |
20.02 |
| 9 |
Stepanek, Livia G (15)
|
NIHAST |
5:31.15 |
5:34.29 |
3.14 |
| 10 |
McNutt, Megan E (18)
|
NIHAWK |
5:23.21 |
5:37.63 |
14.42 |
| 11 |
Sahin, Melis (13)
|
NIPACK |
5:46.96 |
5:55.85 |
8.89 |
| 12 |
Miller, Alayna E (13)
|
NIHAST |
|
5:59.43 |
- |
| 13 |
Salonen-Roperti, Marissa A (16)
|
NIGCOM |
|
5:59.89 |
- |
| 14 |
Reilly, Katie R (14)
|
NIHAWK |
|
6:01.96 |
- |
| 15 |
Bowes, Mara G (14)
|
NIHAWK |
|
6:15.65 |
- |
|
Senglaub, Emma (14)
|
NIVICT |
4:54.36 |
DQ |
- |
|
LeFrois-Heath, Emily R (13)
|
NIVICT |
|
NS |
- |