| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Evans, Mary A (12)
|
NIGRSC |
29.62 |
30.42 |
0.80 |
| 2 |
Caldwell, Hannah M (11)
|
NIUN-V |
31.04 |
31.19 |
0.15 |
| 3 |
Zerger, Belah (12)
|
NIGRSC |
31.93 |
31.37 |
-0.56 |
| 3 |
McKane, Angelina P (11)
|
NIHAWK |
36.00 |
31.37 |
-4.63 |
| 5 |
Zona, Gianna I (12)
|
NIGRSC |
32.48 |
31.46 |
-1.02 |
| 6 |
Tran, Anh Thu D (12)
|
NIHAWK |
32.56 |
32.40 |
-0.16 |
| 7 |
Kazmark, Olivia N (11)
|
NIVICT |
33.07 |
32.81 |
-0.26 |
| 8 |
Hersh, Erin (11)
|
NIVICT |
31.83 |
33.00 |
1.17 |
| 9 |
Martin, Lauren G (12)
|
NISDSC |
|
33.10 |
- |
| 10 |
Bay, Tessa L (11)
|
NIVICT |
35.10 |
33.13 |
-1.97 |
| 11 |
Pierce, Marek J (11)
|
NINEWW |
33.78 |
33.77 |
-0.01 |
| 12 |
Bain, Caitlyn M (11)
|
NIHAWK |
35.02 |
34.74 |
-0.28 |
| 13 |
Childs, Maggie E (12)
|
NIWEBS |
35.85 |
34.79 |
-1.06 |
| 14 |
Hubbard, Mary Elena P (12)
|
NIGRSC |
35.55 |
34.81 |
-0.74 |
| 15 |
Camnitz, Anna M (11)
|
NINEWW |
36.45 |
35.13 |
-1.32 |
| 16 |
Kelley, Michaela G (11)
|
NIGRSC |
35.71 |
35.86 |
0.15 |
| 17 |
Hilker, Audra L (12)
|
NIHAWK |
|
36.51 |
- |
| 18 |
Buck, Kathryn E (12)
|
NIWEBS |
38.29 |
37.29 |
-1.00 |
| 19 |
Kapral, Chloe H (11)
|
NIHAWK |
35.84 |
38.42 |
2.58 |
| 20 |
Stonehouse, Sierra L (12)
|
NIGRSC |
37.09 |
38.69 |
1.60 |
| 21 |
Rouse, Ellie R (12)
|
NIGRAT |
|
39.27 |
- |
| 22 |
Janssen, Delaney A (11)
|
NIWEBS |
41.13 |
39.99 |
-1.14 |
| 23 |
Daly, Violet M (12)
|
NIHAWK |
39.85 |
40.10 |
0.25 |
| 24 |
McCarthy, Kelsey S (11)
|
NIHAWK |
39.64 |
41.18 |
1.54 |
| 25 |
Thurston, Lauren A (11)
|
NIGCOM |
42.14 |
42.75 |
0.61 |
| 26 |
Stoos, Arianna E (11)
|
NIGRAT |
55.24 |
44.01 |
-11.23 |
| 27 |
Saysamone, Leina H (11)
|
NIGCOM |
43.08 |
44.17 |
1.09 |
| 28 |
Richards, Kylie G (11)
|
NIHAWK |
40.14 |
44.38 |
4.24 |
| 29 |
Cady, Makenna N (11)
|
NIWEBS |
48.28 |
46.36 |
-1.92 |
| 30 |
Waters, Megan R (12)
|
NIGCOM |
47.69 |
49.53 |
1.84 |
| 31 |
Raimondo, Erin E (11)
|
NIGRSC |
49.14 |
56.42 |
7.28 |
|
Dibble, Peyton A (11)
|
NIWEBS |
42.04 |
NS |
- |