| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bedford, Marin B (11)
|
NIWEBS |
38.64 |
36.85 |
-1.79 |
| 2 |
Richards, Sydney R (11)
|
NIGRAT |
42.50 |
39.65 |
-2.85 |
| 3 |
Caldwell, Hannah M (12)
|
NIVICT |
41.84 |
39.78 |
-2.06 |
| 4 |
Skizim, Elena K (12)
|
NIWEBS |
39.67 |
41.04 |
1.37 |
| 5 |
Wagner, Morgan E (11)
|
NIVICT |
40.28 |
41.36 |
1.08 |
| 6 |
Wethey, Morgane A (11)
|
NIVICT |
41.84 |
44.08 |
2.24 |
| 7 |
Minnigh, Ella N (12)
|
NIPACK |
48.03 |
46.31 |
-1.72 |
| 8 |
Crisco, Jacqueline E (11)
|
NIHAWK |
49.92 |
46.33 |
-3.59 |
| 9 |
Schwandt, Natalie K (11)
|
NIGRAT |
47.55 |
46.74 |
-0.81 |
| 10 |
Huerta-Andrade, Isabella (12)
|
NIPACK |
48.57 |
47.85 |
-0.72 |
| 11 |
Wagner, Keaghan J (11)
|
NISDSC |
53.27 |
51.94 |
-1.33 |
| 12 |
Downey, Jenna A (11)
|
NINEWW |
55.28 |
53.91 |
-1.37 |
|
Brett, Erin E (11)
|
NIPACK |
45.85 |
NS |
- |