| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Staley, Catherine E (11)
|
NIMERC |
34.71 |
35.50 |
0.79 |
| 2 |
Wethey, Morgane A (11)
|
NIVICT |
34.38 |
35.91 |
1.53 |
| 3 |
Schwandt, Natalie K (11)
|
NIGRAT |
38.90 |
39.41 |
0.51 |
| 4 |
Kapral, Chloe H (12)
|
NIHAWK |
40.63 |
39.45 |
-1.18 |
| 5 |
Riesbeck, Caton S (12)
|
NIHAWK |
44.25 |
40.66 |
-3.59 |
| 6 |
Crisco, Jacqueline E (11)
|
NIHAWK |
43.51 |
41.03 |
-2.48 |
| 7 |
Levi, Noa (11)
|
NIHAWK |
50.70 |
45.63 |
-5.07 |
| 8 |
Nitti, Marissa R (11)
|
NIGRSC |
51.76 |
45.69 |
-6.07 |
| 9 |
Huerta-Andrade, Amanda (11)
|
NIPACK |
48.20 |
46.14 |
-2.06 |
| 10 |
Grigg, Charlotte M (11)
|
NIGRSC |
1:01.46 |
46.64 |
-14.82 |
| 11 |
Close, Kendra A (11)
|
NIHAWK |
50.45 |
48.96 |
-1.49 |
| 12 |
Downey, Jenna A (11)
|
NINEWW |
53.12 |
54.44 |
1.32 |
|
McCarthy, Kelsey S (12)
|
NIHAWK |
49.14 |
NS |
- |
|
Raimondo, Erin E (12)
|
NIGRSC |
|
NS |
- |
|
Brett, Erin E (11)
|
NIPACK |
46.34 |
NS |
- |
|
Nitchman, Melina E (11)
|
NIWEBS |
54.28 |
NS |
- |