| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bartels, Bentley N (9)
|
MDBAY |
46.03 |
45.59 |
-0.44 |
| 2 |
Schweikert, Grant M (8)
|
MDASC |
|
48.42 |
- |
| 3 |
Guyer, Dempsey Q (9)
|
MDASC |
51.77 |
49.15 |
-2.62 |
| 4 |
Tookey, Tate E (10)
|
MDASC |
49.69 |
51.53 |
1.84 |
| 5 |
Cohen, Joshua A (9)
|
MDBAY |
56.37 |
52.50 |
-3.87 |
| 6 |
Casillo, Kyle A (10)
|
MDASC |
|
53.62 |
- |
| 7 |
Jackson, Quincy T (10)
|
MDEST |
57.74 |
53.92 |
-3.82 |
| 8 |
Liang, Jason W (10)
|
MDRAC |
|
54.19 |
- |
| 9 |
Wittig, Bryce R (9)
|
MDBAY |
1:01.10 |
55.95 |
-5.15 |
| 10 |
Smit, Charles M (9)
|
MDASC |
|
55.97 |
- |
| 11 |
Smit, James M (9)
|
MDASC |
|
58.69 |
- |
| 12 |
Dean, Brady M (9)
|
MDRAC |
|
1:00.09 |
- |
| 13 |
Saxton, Kellen M (9)
|
MDEST |
|
1:00.11 |
- |
| 14 |
Heckman, Sawyer W (10)
|
MDASC |
|
1:03.42 |
- |
| 15 |
Flood, Benjamin M (10)
|
MDEST |
1:06.22 |
1:09.34 |
3.12 |
|
Vernieres, Ludovic K (10)
|
MDASC |
|
DQ |
- |
|
Pitts, Brayden P (9)
|
MDBAY |
1:11.98 |
DQ |
- |
|
Edwards, Everett D (9)
|
MDASC |
1:11.90 |
DQ |
- |