| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Purdy, Alexander J (12)
|
WTUN |
54.40 |
55.65 |
1.25 |
| 2 |
Yount, Cody A (12)
|
STNTRO |
58.08 |
55.91 |
-2.17 |
| 3 |
Liang, Ben (11)
|
STNTRO |
56.40 |
56.03 |
-0.37 |
| 4 |
Landry, Zavin L (12)
|
NTNTN |
56.48 |
56.79 |
0.31 |
| 5 |
Lee, Kyle L (11)
|
STNTRO |
1:01.74 |
57.68 |
-4.06 |
| 6 |
Heldenbrand, Dylan F (12)
|
NTNTN |
57.93 |
58.46 |
0.53 |
| 7 |
Chu, Paul N (12)
|
NTCOPS |
1:01.72 |
59.36 |
-2.36 |
| 8 |
Nguyen, Philippe D (12)
|
NTCOPS |
55.97 |
59.40 |
3.43 |
| 9 |
Ruhl, Jonah W (11)
|
NTATAC |
59.71 |
59.46 |
-0.25 |
| 10 |
Tong, Elijah C (12)
|
NTTFA |
1:00.10 |
59.79 |
-0.31 |
| 11 |
Stewart, Joshua W (12)
|
NTPAC |
1:00.34 |
59.94 |
-0.40 |
| 12 |
Isaacs-Sodeye, Judah I (12)
|
NTNTN |
1:01.77 |
1:00.34 |
-1.43 |
| 13 |
Duran De La Vega, Pablo (12)
|
NTMTRO |
1:00.79 |
1:00.56 |
-0.23 |
| 14 |
Gomez, Miguel L (11)
|
STNTRO |
1:02.89 |
1:00.68 |
-2.21 |
| 15 |
Liberi, Gavin R (11)
|
NTDM |
1:01.25 |
1:01.01 |
-0.24 |
| 16 |
Dickheiser, Kobe Y (11)
|
NTNTN |
1:03.71 |
1:01.09 |
-2.62 |
| 17 |
Hargraves, Brady D (12)
|
NTMTRO |
1:02.81 |
1:02.00 |
-0.81 |
| 18 |
Kang, Elijah H (11)
|
STNTRO |
1:04.43 |
1:02.10 |
-2.33 |
| 19 |
Jakhangirov, Boburkhan * (11)
|
STNTRO |
1:04.30 |
1:03.22 |
-1.08 |
| 20 |
Scott, Henry S (12)
|
NTCOR |
1:04.76 |
1:03.34 |
-1.42 |