| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boyer, Chloe G (8)
|
NIISWM |
41.39 |
38.92 |
-2.47 |
| 2 |
El Ghissassi, Sidra S (7)
|
NIVEST |
42.78 |
43.15 |
0.37 |
| 3 |
Ramos, Daniela I (8)
|
NIUNAC |
45.24 |
43.57 |
-1.67 |
| 4 |
Leonard, Kaitllyn S (8)
|
NIWAAC |
1:06.08 |
50.30 |
-15.78 |
| 5 |
Wang, Bethany B (6)
|
NIKRKN |
|
51.55 |
- |
| 6 |
Blakely-Armitage, Fiona S (8)
|
NIISWM |
|
52.34 |
- |
| 7 |
Leahey, Emme A (8)
|
NIUNAC |
|
56.82 |
- |
| 8 |
Chandler, Nora M (7)
|
NICVSC |
|
58.08 |
- |
| 9 |
Williams, Julia M (7)
|
NISKAN |
|
59.50 |
- |
| 10 |
Jefferis, Frida R (8)
|
NIISWM |
|
59.98 |
- |
| 11 |
Tyler, Natalie L (7)
|
NICVSC |
|
1:00.86 |
- |
| 12 |
Roberts, Olivia M (8)
|
NIHAWK |
|
1:04.00 |
- |
| 13 |
Knowlton, Ava L (8)
|
NIKRKN |
|
1:06.65 |
- |
| 14 |
Williams, Lauren K (8)
|
NIHAWK |
|
1:07.15 |
- |
| 15 |
Spencer, Danielle H (6)
|
NIWAAC |
2:20.90 |
1:33.81 |
-47.09 |