| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Craig, Molly C (18)
|
NIKRKN |
2:29.27 |
2:28.16 |
-1.11 |
| 2 |
Craig, Catherine K (16)
|
NIKRKN |
2:34.50 |
2:35.58 |
1.08 |
| 3 |
Pierce, Summer M (15)
|
NIKRKN |
2:39.47 |
2:39.29 |
-0.18 |
| 4 |
Schultz, Katie (17)
|
NICVSC |
2:34.39 |
2:43.55 |
9.16 |
| 5 |
Bernhardt, Leah A (15)
|
NICORY |
2:55.78 |
2:44.63 |
-11.15 |
| 6 |
Incitti, Iris C (17)
|
NIUNAC |
2:41.42 |
2:46.21 |
4.79 |
| 7 |
Grunder, Hannah (17)
|
NICAGO |
2:52.36 |
2:50.83 |
-1.53 |
| 8 |
Olzinski, Molly E (16)
|
NIUNAC |
3:00.91 |
2:54.61 |
-6.30 |
| 9 |
Harford, Starr V (15)
|
NIWAAC |
3:05.81 |
2:55.67 |
-10.14 |
| 10 |
Ludlow, Allison G (18)
|
NIMERC |
2:43.63 |
2:57.86 |
14.23 |
| 11 |
Kittle, Kayla A (15)
|
NIWAAC |
3:11.45 |
2:58.33 |
-13.12 |
| 12 |
Christensen, Sydney L (15)
|
NIUNAC |
2:57.94 |
2:58.37 |
0.43 |
| 13 |
Twarkusky, Haley R (17)
|
NIWAAC |
|
2:58.53 |
- |
| 14 |
Coombs, Rachel E (17)
|
NICORY |
2:56.73 |
3:00.63 |
3.90 |
| 15 |
TenKate, Caroline J (15)
|
NICORY |
|
3:03.10 |
- |
| 16 |
Keefer, Lillian (16)
|
NIWAAC |
|
3:07.43 |
- |
| 17 |
Chermak, Claudia R (15)
|
NIUNAC |
3:03.51 |
3:08.61 |
5.10 |
| 18 |
Christensen, Taylor J (17)
|
NIUNAC |
|
3:08.86 |
- |
| 19 |
Gorsline, Kendra L (17)
|
NIWAAC |
3:22.59 |
3:09.98 |
-12.61 |
| 20 |
Bowman, Marah E (16)
|
NIUNAC |
3:20.02 |
3:18.72 |
-1.30 |
| 21 |
Bard, Christina A (15)
|
NIWAAC |
|
3:24.21 |
- |
|
Williamson, Abigail J (15)
|
NIISWM |
3:16.98 |
DQ |
- |