| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boyer, Chloe G (9)
|
NIISWM |
41.39 |
34.21 |
-7.18 |
| 2 |
Ramos, Daniela I (9)
|
NIUNAC |
43.57 |
37.51 |
-6.06 |
| 3 |
Melveney, Emily R (10)
|
NIGGST |
38.00 |
39.91 |
1.91 |
| 4 |
Ryan, Natalie C (9)
|
NIUNAC |
47.80 |
42.00 |
-5.80 |
| 5 |
Denlinger, Elizabeth J (10)
|
NIWAAC |
|
43.28 |
- |
| 6 |
Leonard, Kaitllyn S (9)
|
NIWAAC |
1:06.08 |
44.94 |
-21.14 |
| 7 |
Mitchell, Hope J (9)
|
NIGGST |
50.00 |
45.06 |
-4.94 |
| 8 |
Blakely-Armitage, Fiona S (9)
|
NIISWM |
49.66 |
45.22 |
-4.44 |
| 9 |
Leahey, Emme A (9)
|
NIUNAC |
56.82 |
45.33 |
-11.49 |
| 10 |
Vanderhoof, Emma J (10)
|
NIWAAC |
|
46.70 |
- |
| 11 |
Roberts, Olivia M (9)
|
NIHAWK |
55.90 |
55.48 |
-0.42 |
| 12 |
Williams, Lauren K (9)
|
NIHAWK |
59.56 |
57.48 |
-2.08 |