| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hall, Sawyer J (15)
|
NIVICT |
27.49 |
27.75 |
0.26 |
| 2 |
Nau, Zachary T (17)
|
NIHAST |
28.59 |
27.82 |
-0.77 |
| 3 |
Zani, Samuel A (15)
|
NIIRON |
28.71 |
28.56 |
-0.15 |
| 4 |
Riexinger, Colby J (15)
|
NISEAS |
29.15 |
29.36 |
0.21 |
| 5 |
Morreale, Alexander H (14)
|
NIHAST |
29.13 |
29.38 |
0.25 |
| 6 |
Dinh, Benson (14)
|
NIGRSC |
29.76 |
30.16 |
0.40 |
| 7 |
Murray, Maxwell P (15)
|
NISDSC |
29.35 |
30.27 |
0.92 |
| 8 |
Riexinger, Paul M (44)
|
NISEAS |
31.90 |
31.58 |
-0.32 |
| 9 |
Hahn, Kurt C (13)
|
NISDSC |
30.71 |
31.68 |
0.97 |
| 10 |
DiFante, Mitchell T (13)
|
NISEAS |
31.54 |
32.22 |
0.68 |
| 11 |
Cunningham, Dalton M (13)
|
NISEAS |
30.50 |
32.46 |
1.96 |
| 12 |
Haibach, Tristan J (14)
|
NISEAS |
36.95 |
37.92 |
0.97 |
| 13 |
Peters, Ryan R (13)
|
NIGCOM |
42.52 |
41.14 |
-1.38 |
|
Ciravola, Jayden T (14)
|
NIHAST |
34.04 |
NS |
- |
|
Manhertz, Jalen K (14)
|
NIIRON |
33.32 |
NS |
- |