| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guilfoil, Ella K (12)
|
NIFAST |
30.78 |
30.18 |
-0.60 |
| 2 |
DeHollander, Erin K (12)
|
NIVICT |
31.58 |
31.15 |
-0.43 |
| 3 |
Cropper, Iala K (12)
|
NIFAST |
33.68 |
34.52 |
0.84 |
| 4 |
Kazmark, Raelyn J (12)
|
NIVICT |
36.38 |
35.57 |
-0.81 |
| 5 |
Quigley, Brienne K (12)
|
NIGRSC |
35.45 |
35.76 |
0.31 |
| 6 |
Maynard, Chelsea A (11)
|
NISDSC |
35.22 |
35.84 |
0.62 |
| 7 |
Barry, Madeline G (11)
|
NIVICT |
37.29 |
37.26 |
-0.03 |
| 8 |
Kazmark, Olivia N (11)
|
NIVICT |
36.90 |
38.19 |
1.29 |
| 9 |
Carey, Madison H (12)
|
NIIRON |
38.15 |
39.69 |
1.54 |
| 10 |
Nguessamble, Tristyn D (11)
|
NISDSC |
45.51 |
41.22 |
-4.29 |
| 11 |
Robinson, Sara C (11)
|
NISDSC |
42.75 |
42.81 |
0.06 |
| 12 |
Campbell, Brooklyn M (11)
|
NIVICT |
42.65 |
43.94 |
1.29 |
| 13 |
Armet, Emma E (11)
|
NIOLSC |
48.15 |
44.41 |
-3.74 |
| 14 |
Osborne, Samantha R (11)
|
NIGRSC |
44.40 |
44.44 |
0.04 |
|
Evans, Mary A (12)
|
NIGRSC |
35.53 |
NS |
- |
|
Werges, Lillian F (12)
|
NIVICT |
33.22 |
NS |
- |