| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rodriguez, Caroline (15)
|
FGASC |
26.39 |
26.28 |
-0.11 |
| 2 |
Koch, Ellie (15)
|
FGYSF |
25.62 |
26.58 |
0.96 |
| 3 |
Riley, Elizabeth M (15)
|
FGYSF |
26.18 |
26.91 |
0.73 |
| 4 |
Gonoretzky, Jacqueline (16)
|
FGUN-F |
26.55 |
26.95 |
0.40 |
| 5 |
Kelly, Jemma H (13)
|
FGYSF |
26.98 |
27.03 |
0.05 |
| 6 |
Ravelo, Iliana (14)
|
FGASC |
27.46 |
27.05 |
-0.41 |
| 7 |
Adia, Isabella I (13)
|
FGUN |
29.36 |
29.17 |
-0.19 |
| 8 |
Nemeth, Emese g (16)
|
FGPAQ |
29.34 |
29.30 |
-0.04 |
| 9 |
Conte, Holley A (13)
|
FGECAC |
29.81 |
29.56 |
-0.25 |
| 10 |
Gouverneur, Laura S (15)
|
FGYSF |
29.35 |
29.63 |
0.28 |
| 11 |
Albuquerque, Esther O (13)
|
FGPAQ |
29.68 |
29.72 |
0.04 |
| 12 |
O'Leary, Olivia V (13)
|
FGFAST |
30.06 |
29.80 |
-0.26 |
| 13 |
Nguyen, Alexis (13)
|
FGSAS |
31.16 |
30.03 |
-1.13 |
| 14 |
Klapper, Giulia f (13)
|
FGPAQ |
28.81 |
30.38 |
1.57 |
| 15 |
Feliciano, Sarah G (16)
|
FGPAQ |
29.86 |
30.45 |
0.59 |
| 16 |
Gouverneur, Isabelle K (17)
|
FGYSF |
29.96 |
30.63 |
0.67 |
| 17 |
Canning, Caroline R (13)
|
FGPAQ |
31.45 |
31.30 |
-0.15 |
| 18 |
Zuniga, Isabella M (16)
|
FGPAQ |
29.89 |
32.60 |
2.71 |
| 18 |
Rossi, Kathleen R (13)
|
FGSAS |
32.84 |
32.60 |
-0.24 |
| 20 |
Rodriguez, Victoria (15)
|
FGASC |
32.39 |
32.67 |
0.28 |
| 21 |
Agnello, Taylor N (14)
|
FGFAST |
32.58 |
33.02 |
0.44 |
| 22 |
Kotegova, Maria (13)
|
FGPAQ |
32.27 |
33.39 |
1.12 |
| 23 |
Gomez-Canas, Juliana R (13)
|
FGBMSC |
33.76 |
35.45 |
1.69 |
| 24 |
Al-Salem, Anna O (17)
|
FGPAQ |
34.06 |
35.60 |
1.54 |
| 25 |
Maradiagla, Gabryella N (13)
|
FGLLL |
40.56 |
41.98 |
1.42 |
| 26 |
Rosier, Tamar E (15)
|
FGLLL |
|
48.37 |
- |
| 27 |
Goodwin, Sophia M (15)
|
FGLLL |
47.46 |
49.14 |
1.68 |
|
Escobar, Elizabeth M (13)
|
FGBMSC |
|
NS |
- |
|
Akdag, Julie P (13)
|
FGLLL |
41.13 |
NS |
- |
|
Escobar, Gina T (13)
|
FGBMSC |
|
NS |
- |