| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Atkins, Alania M (11)
|
NCEAC |
36.27 |
36.51 |
0.24 |
| 2 |
Trevino, Gabriella B (12)
|
NCEAC |
38.37 |
36.78 |
-1.59 |
| 3 |
Starkey, Kristen N (12)
|
NCEAC |
38.23 |
39.07 |
0.84 |
| 4 |
Johnston, Conley E (11)
|
NCEAC |
39.82 |
40.23 |
0.41 |
| 5 |
Kaczorowski, Caitlyn A (12)
|
NCNEAL |
47.84 |
42.25 |
-5.59 |
| 6 |
Pate, Danielle A (11)
|
NCNEAL |
50.07 |
42.76 |
-7.31 |
| 7 |
Brundage, Taylor K (11)
|
NCEAC |
|
43.77 |
- |
| 8 |
Holcomb, Amelia B (11)
|
NCEAC |
46.59 |
44.59 |
-2.00 |
| 9 |
Milkowich, Alayna G (12)
|
NCNEAL |
43.63 |
45.03 |
1.40 |
| 10 |
Spurrier, Elle M (11)
|
NCEAC |
47.60 |
45.71 |
-1.89 |
| 11 |
Marino, Gabriella E (11)
|
NCNEAL |
48.23 |
46.03 |
-2.20 |
| 12 |
Walker, Collins E (12)
|
NCNEAL |
49.91 |
46.44 |
-3.47 |
| 13 |
Moore, Olivia J (12)
|
NCEAC |
45.99 |
48.48 |
2.49 |
| 14 |
Fougner, Jalynn R (12)
|
NCNEAL |
51.13 |
49.83 |
-1.30 |
| 15 |
Wilson, Julie (11)
|
NCNEAL |
52.37 |
51.63 |
-0.74 |
| 16 |
Byrd, Amia L (12)
|
NCUN-1 |
|
54.51 |
- |
| 17 |
Pope, Aubrey F (11)
|
NCNEAL |
1:10.18 |
57.38 |
-12.80 |
| 18 |
Deguire, Brinley M (11)
|
NCEAC |
1:00.99 |
59.05 |
-1.94 |
|
Quinlan, Ava (12)
|
NCNEAL |
42.93 |
DQ |
- |
|
Abernathy, Ella J (11)
|
NCEAC |
44.89 |
NS |
- |
|
Piemonte, Honor E (12)
|
NCPAST |
47.94 |
NS |
- |