| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Foltz, Caroline A (13)
|
MAYY |
2:39.25 |
2:33.18 |
-6.07 |
| 2 |
Doss, Gabrielle H (14)
|
MAGSVY |
2:23.96 |
2:34.19 |
10.23 |
| 3 |
Liu, Madeleine P (14)
|
NJWFY |
2:31.88 |
2:35.53 |
3.65 |
| 4 |
Welker, Sydney E (14)
|
MAYY |
2:39.02 |
2:35.80 |
-3.22 |
| 5 |
Leydig, Camryn G (13)
|
MAYY |
2:48.21 |
2:41.50 |
-6.71 |
| 6 |
Decker, Alexandra G (14)
|
MAGSVY |
2:39.01 |
2:44.03 |
5.02 |
| 7 |
Strayer, Catherine (14)
|
MAYY |
2:49.30 |
2:44.40 |
-4.90 |
| 8 |
Grove, Emma (13)
|
MACC |
2:56.56 |
2:46.67 |
-9.89 |
| 9 |
Groff, Avery (14)
|
MAYY |
|
2:49.39 |
- |
| 10 |
McCombs, Emma C (14)
|
MAYY |
2:51.46 |
2:49.73 |
-1.73 |
| 11 |
Speed, Deanna L (13)
|
MAYY |
2:56.75 |
2:51.18 |
-5.57 |
| 12 |
Baker, Kasie L (14)
|
MDYCM |
2:47.29 |
2:51.54 |
4.25 |
| 13 |
Parks, Kylee E (14)
|
MAHAY |
2:56.46 |
2:53.19 |
-3.27 |
| 14 |
Heiner, Brooke A (14)
|
MAYY |
2:55.50 |
2:53.99 |
-1.51 |
| 15 |
Augustin, Tadem S (13)
|
MDYCM |
3:24.28 |
2:54.37 |
-29.91 |
| 16 |
Terrazas, Olivia (13)
|
MACC |
|
2:56.12 |
- |
| 17 |
Hoffmann, Claire C (14)
|
NJWFY |
2:56.39 |
2:56.67 |
0.28 |
| 18 |
Park, Laura (14)
|
NJWFY |
2:55.22 |
2:57.90 |
2.68 |
| 19 |
Calder, Ella C (13)
|
MAYY |
3:02.71 |
2:58.25 |
-4.46 |
| 20 |
Horkan, Kathryn E (13)
|
NJWFY |
3:00.00 |
2:59.21 |
-0.79 |
| 21 |
Kahsay, Victoria (14)
|
MACC |
|
3:09.19 |
- |
| 22 |
Wilson, Maggie E (13)
|
NJWFY |
2:50.00 |
3:13.44 |
23.44 |
| 23 |
Crippa, Alessandra (13)
|
MDYCM |
|
3:16.99 |
- |
| 24 |
Rowan, Madeline A (13)
|
MAGSVY |
|
3:28.99 |
- |
|
Kapp, Christine O (13)
|
MAYY |
2:47.48 |
DQ |
- |
|
Treeman-Reinke, Ariadne I (14)
|
MDYCM |
3:13.65 |
NS |
- |
|
Mahoney, Breyanna D (13)
|
MDYCM |
|
NS |
- |
|
Brandenburg, Grace H (13)
|
MDYCM |
|
NS |
- |