| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Younica, Lillian A (10)
|
VA757 |
33.97 |
34.77 |
0.80 |
| 2 |
Hoadley, Addison G (10)
|
VA757 |
52.53 |
41.70 |
-10.83 |
| 3 |
Li, Chelsea T (8)
|
VACVAC |
42.91 |
41.94 |
-0.97 |
| 4 |
Gideon, Whitney S (10)
|
VACVAC |
39.68 |
42.09 |
2.41 |
| 5 |
Landon, Sarah K (9)
|
VACVAC |
43.44 |
44.53 |
1.09 |
| 6 |
Henshaw, Emerson F (10)
|
VA757 |
43.41 |
44.83 |
1.42 |
| 7 |
Tracey, Mary G (10)
|
VA757 |
42.40 |
45.95 |
3.55 |
| 8 |
Roitsch, Reagan E (9)
|
VACVAC |
|
46.93 |
- |
| 9 |
Mueller, Taylor N (9)
|
VACGBD |
45.90 |
47.58 |
1.68 |
| 10 |
Stevens, Callie G (8)
|
VACGBD |
|
47.75 |
- |
| 11 |
Daniels, Isabelle K (8)
|
VACVAC |
|
53.51 |
- |
| 12 |
Raeburn, Morgan Delaney (8)
|
VACVAC |
54.24 |
55.37 |
1.13 |
| 13 |
Ericsson, Lindsey J (9)
|
VA757 |
36.11 |
56.30 |
20.19 |
| 15 |
Hamilton, Keller R (9)
|
VA757 |
55.99 |
58.21 |
2.22 |
| 16 |
Malnati, Eliana C (10)
|
VACVAC |
|
1:07.46 |
- |
|
Schwarzenberg-Word, Alia (8)
|
VACGBD |
|
NS |
- |
|
Trant, Julia M (9)
|
VA757 |
|
NS |
- |