| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Beaman, Katherine L (9)
|
VAECAT |
53.19 |
52.76 |
-0.43 |
| 2 |
Beaman, Elizabeth G (9)
|
VAECAT |
54.99 |
56.98 |
1.99 |
| 3 |
Henderson, Kiera L (10)
|
VACGBD |
1:06.00 |
59.19 |
-6.81 |
| 4 |
Shipe, Chloe E (9)
|
VACGBD |
59.56 |
59.71 |
0.15 |
| 5 |
Sturgill, Claire E (10)
|
VACGBD |
|
1:01.49 |
- |
| 6 |
Powers, Gabriella J (10)
|
VACGBD |
|
1:03.73 |
- |
| 7 |
Wood, Abby S (8)
|
VACGBD |
|
1:03.99 |
- |
| 8 |
Murphy, Morgan L (9)
|
VACGBD |
|
1:04.19 |
- |
| 9 |
Condon, Keely R (10)
|
VA757 |
|
1:04.55 |
- |
| 10 |
Gardner, Olivia E (9)
|
VAECAT |
|
1:09.60 |
- |
| 11 |
Angel, Layla G (10)
|
VACVAC |
|
1:26.29 |
- |
|
Beckhusen Challener, Annalis (9)
|
VA757 |
|
DQ |
- |
|
Condon, Cora B (9)
|
VA757 |
|
DQ |
- |
|
Creamer, Gabrielle J (10)
|
VACVAC |
|
DQ |
- |