| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baker, Haley K (8)
|
VAUN |
41.40 |
38.90 |
-2.50 |
| 2 |
Mitchell, Madison (8)
|
VAACAC |
41.33 |
44.52 |
3.19 |
| 3 |
Gantz, Kara E (8)
|
VAUN |
47.06 |
44.57 |
-2.49 |
| 4 |
Holt, Bethany A (8)
|
VAACAC |
47.43 |
44.71 |
-2.72 |
| 5 |
Malatesta, Isabelle R (8)
|
VARACE |
|
45.15 |
- |
| 6 |
Williams, Mallory Grace N (8)
|
VA757 |
43.89 |
45.27 |
1.38 |
| 7 |
Spaulding, Kaitlyn K (8)
|
VA757 |
|
46.05 |
- |
| 8 |
Bontrager, Peyton R (8)
|
VASRVA |
|
47.62 |
- |
| 9 |
Barnes, Reagan M (8)
|
VA757 |
|
48.78 |
- |
| 10 |
Rogers, Makenzie A (7)
|
VASRVA |
|
49.53 |
- |
| 11 |
Davis, Eleanor J (8)
|
VA757 |
55.67 |
50.46 |
-5.21 |
| 12 |
Hoadley, Addison G (7)
|
VA757 |
|
53.48 |
- |
| 13 |
Maynard, Addison (6)
|
VAACAC |
1:05.33 |
53.87 |
-11.46 |
| 14 |
Keithley, Jennings C (8)
|
VA757 |
|
57.86 |
- |
| 15 |
Ananthram, Jaya I (7)
|
VA757 |
|
58.48 |
- |
| 16 |
Spaulding, Emmalyn M (6)
|
VA757 |
|
1:07.58 |
- |
|
Smith, Madelynn E (6)
|
VAUN |
1:17.74 |
NS |
- |