| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Long, Sofia R (18)
|
VA757 |
55.14 |
58.42 |
3.28 |
| 2 |
Hurt, Colby K (18)
|
VA757 |
56.28 |
59.37 |
3.09 |
| 3 |
Epes, Ella M (15)
|
VA757 |
56.96 |
1:00.27 |
3.31 |
| 4 |
Sullivan, Kaitlyn C (16)
|
VA757 |
58.25 |
1:01.13 |
2.88 |
| 5 |
Maly, Jacquelyn M (19)
|
VA757 |
58.51 |
1:01.31 |
2.80 |
| 6 |
Lynch, Virginia N (17)
|
VA757 |
1:00.07 |
1:03.16 |
3.09 |
| 7 |
Lederer, Abigail J (15)
|
VACVAC |
1:01.20 |
1:03.60 |
2.40 |
| 8 |
Johnson, Kylie M (17)
|
VA757 |
1:01.51 |
1:03.87 |
2.36 |
| 9 |
Mosley, Kristilynn E (15)
|
VATIDE |
1:01.29 |
1:04.29 |
3.00 |
| 10 |
Wojtkowski, Erin P (18)
|
VASEVA |
1:06.71 |
1:06.54 |
-0.17 |
| 11 |
Hudgins, Dagny R (17)
|
VASEVA |
1:03.32 |
1:07.17 |
3.85 |
| 12 |
Inskeep, Caroline L (16)
|
VACVAC |
1:05.90 |
1:07.45 |
1.55 |
| 13 |
Schooneman, Kim (16)
|
VATIDE |
1:09.82 |
1:08.21 |
-1.61 |
| 14 |
White, Jenna C (15)
|
VASEVA |
1:09.17 |
1:14.32 |
5.15 |
| 15 |
Tarantino, Grace K (18)
|
VA757 |
1:09.73 |
1:16.05 |
6.32 |
| 16 |
Monestine, Ymanee R (15)
|
VA757 |
1:13.65 |
1:17.12 |
3.47 |
| 17 |
Saddler, Haylee T (15)
|
VATIDE |
1:23.00 |
1:26.63 |
3.63 |
| 18 |
Buzolich, Isabelle R (16)
|
VATIDE |
1:37.54 |
1:39.27 |
1.73 |