| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dietrich, Stella R (8)
|
VATIDE |
25.51 |
22.38 |
-3.13 |
| 2 |
Heustis, Bailey M (8)
|
VAODAC |
22.26 |
22.72 |
0.46 |
| 3 |
Guzman, Maite A (8)
|
VATIDE |
27.84 |
23.38 |
-4.46 |
| 4 |
Culicerto, June C (8)
|
VATIDE |
22.99 |
23.82 |
0.83 |
| 5 |
Ervin, Joslyn E (8)
|
VATIDE |
24.12 |
24.19 |
0.07 |
| 6 |
Yancey, Avery E (8)
|
VAODAC |
26.09 |
25.97 |
-0.12 |
| 7 |
Dela Cruz, Rianna Almira V (8)
|
VATIDE |
25.99 |
26.22 |
0.23 |
| 8 |
Eberle, Lisa M (8)
|
VATIDE |
30.31 |
26.30 |
-4.01 |
| 9 |
Seniuk, Rose E (8)
|
VATIDE |
27.76 |
26.78 |
-0.98 |
| 10 |
Mannato, Alexis L (8)
|
VATIDE |
|
26.94 |
- |
| 11 |
Niederberger, Keely M (8)
|
VATIDE |
28.63 |
28.62 |
-0.01 |
| 12 |
Poul, Bailey E (8)
|
VAODAC |
39.97 |
29.53 |
-10.44 |
| 13 |
Krell, Riley A (8)
|
VAECAT |
27.38 |
30.00 |
2.62 |
| 14 |
Hart, Hillary A (8)
|
VAODAC |
31.78 |
30.12 |
-1.66 |
| 15 |
Maisenbacher, Elise K (8)
|
VATIDE |
26.75 |
30.38 |
3.63 |
| 16 |
Varner, Elaina E (8)
|
VAECAT |
33.63 |
30.74 |
-2.89 |
| 17 |
Gaston, Rachel E (8)
|
VATAC |
30.03 |
32.40 |
2.37 |
| 18 |
Smith, Arilynn L (8)
|
VAODAC |
33.00 |
32.50 |
-0.50 |
| 19 |
Jamba, Cobie E (8)
|
VAECAT |
37.44 |
36.25 |
-1.19 |
| 20 |
Maurer, Abby A (8)
|
VAODAC |
37.63 |
39.85 |
2.22 |
|
Chester, Peyton B (8)
|
VATIDE |
|
NS |
- |
|
Aquino, Meagan E (8)
|
VAODAC |
23.49 |
NS |
- |