| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Viera, Isabel (10)
|
FGMACM |
36.60 |
35.37 |
-1.23 |
| 2 |
Viera, Andrea (10)
|
FGMACM |
40.28 |
38.89 |
-1.39 |
| 3 |
Karachun, Daryna (10)
|
FGYSF |
39.15 |
39.54 |
0.39 |
| 4 |
Montenegro, Makayla E (9)
|
FGMACM |
41.46 |
41.16 |
-0.30 |
| 5 |
Di Sarno, Naiyla F (9)
|
FGMWA |
40.79 |
41.36 |
0.57 |
| 6 |
Suarez, Ava B (10)
|
FGYSF |
43.67 |
43.85 |
0.18 |
| 7 |
Altamura, Benedetta R (10)
|
FGPST |
44.11 |
44.08 |
-0.03 |
| 8 |
Chacon, Kayla M (10)
|
FGSF |
|
46.11 |
- |
| 9 |
Roldan, Arianna * (9)
|
FGGRSC |
45.55 |
46.15 |
0.60 |
| 10 |
Moreno, Antonia (9)
|
FGGRSC |
47.48 |
49.37 |
1.89 |
| 11 |
Ramos Miret, Carolina (9)
|
FGMACM |
|
49.56 |
- |
| 12 |
Souza, Gabriela L (10)
|
FGGRSC |
52.40 |
50.94 |
-1.46 |
| 13 |
Dabus, Rafaela A (9)
|
FGGRSC |
1:00.95 |
55.34 |
-5.61 |
| 14 |
Prigmore, Lauren (9)
|
FGMWA |
|
1:01.93 |
- |
| 15 |
Lenz Cesar, Maria Luiza B (9)
|
FGGRSC |
|
1:02.42 |
- |
|
Rodriguez, Roxan (10)
|
FGMWA |
|
DQ |
- |
|
Chaison, Molly A (10)
|
FGMWA |
45.29 |
NS |
- |