| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rossi, Angelina G (16)
|
OKAESC |
34.43 |
35.36 |
0.93 |
| 2 |
Holt, Sarah E (16)
|
LARRAC |
39.65 |
38.24 |
-1.41 |
| 3 |
Gordon, Mary-Ashlynne G (16)
|
GUCATS |
40.54 |
39.80 |
-0.74 |
| 4 |
Oliphant, Amaya C (15)
|
OKJTSC |
42.39 |
39.86 |
-2.53 |
| 5 |
Zhang, Evelyn L (15)
|
LARRAC |
41.64 |
41.31 |
-0.33 |
| 6 |
Richardson, Alista M (16)
|
OKBSC |
|
41.63 |
- |
| 7 |
Vo, Justina A (15)
|
LARRAC |
43.31 |
43.73 |
0.42 |
| 8 |
Faulkner, Katherine A (15)
|
GUCATS |
42.37 |
44.28 |
1.91 |
| 9 |
Scaglione, Faith A (15)
|
MVANDR |
|
47.57 |
- |
| 10 |
Lichti, Abigail C (15)
|
OKAESC |
50.69 |
58.20 |
7.51 |