| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gordon, Mary-Ashlynne G (16)
|
GUCATS |
4:39.64 |
4:36.94 |
-2.70 |
| 2 |
Neugent, Natalie A (15)
|
MVANDR |
4:54.70 |
4:56.47 |
1.77 |
| 3 |
Agee, Hannah C (15)
|
OKSSC |
5:15.42 |
4:59.74 |
-15.68 |
| 4 |
Faulkner, Katherine A (15)
|
GUCATS |
4:55.00 |
5:01.99 |
6.99 |
| 5 |
Monroe, Madysen J (16)
|
WTAST |
5:21.89 |
5:09.20 |
-12.69 |
| 6 |
Parker, Krista N (15)
|
OKKMS |
5:11.93 |
5:10.97 |
-0.96 |
| 7 |
Richardson, Alista M (16)
|
OKBSC |
5:04.12 |
5:21.49 |
17.37 |
| 8 |
Ward, Kaylee B (15)
|
OKTAC |
5:13.03 |
5:26.90 |
13.87 |
| 9 |
Rau, Jasmine T (15)
|
OKJTSC |
5:42.79 |
5:30.06 |
-12.73 |
| 10 |
Smith, Kaya N (15)
|
OKJTSC |
5:24.64 |
5:35.62 |
10.98 |
| 11 |
White, Ariana G (16)
|
OKJTSC |
5:36.46 |
5:52.29 |
15.83 |
| 12 |
Scaglione, Faith A (15)
|
MVANDR |
6:12.14 |
5:53.44 |
-18.70 |
| 13 |
Aleman, Tyler S (15)
|
OKJTSC |
|
6:26.39 |
- |