| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liedka, Ryan J (12)
|
NIMETS |
41.22 |
38.69 |
-2.53 |
| 2 |
Smith, Keenan (12)
|
NIBSSC |
41.56 |
40.17 |
-1.39 |
| 3 |
Kessler, Scott M (12)
|
NIMETS |
43.79 |
43.20 |
-0.59 |
| 4 |
Miller, Treyse B (11)
|
NIMETS |
46.32 |
44.77 |
-1.55 |
| 5 |
Anthony, Jaydon T (11)
|
NIPMSC |
46.11 |
45.32 |
-0.79 |
| 6 |
Nelson, Payton J (11)
|
NIMETS |
48.37 |
47.83 |
-0.54 |
| 7 |
Stevens, Jonathan P (11)
|
NIJETS |
51.58 |
49.98 |
-1.60 |
| 8 |
Laws, Clifford R (12)
|
NIJETS |
48.85 |
50.48 |
1.63 |
| 9 |
Thompson, Riley J (12)
|
NIOLSC |
56.91 |
55.28 |
-1.63 |
| 10 |
Gates, Jacob J (11)
|
NIMETS |
53.13 |
56.02 |
2.89 |
| 11 |
Oakes, Avery D (11)
|
NIMETS |
58.52 |
57.43 |
-1.09 |
| 12 |
Glidden, Nathan B (11)
|
NIBSSC |
58.23 |
1:00.28 |
2.05 |
| 13 |
Andrews, Alec J (11)
|
NIBSSC |
58.82 |
1:03.06 |
4.24 |
| 14 |
Chavez, Julian L (12)
|
NINAYE |
1:30.00 |
1:19.99 |
-10.01 |
|
Lange, Matthew (11)
|
NIBSSC |
53.68 |
DQ |
- |
|
Denkenberger, Quinten M (11)
|
NIOLSC |
|
DQ |
- |