| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Constantin, Mia (14)
|
VAGATR |
41.76 |
37.40 |
-4.36 |
| 2 |
Rinald, Megan E (14)
|
VAACAC |
42.48 |
39.76 |
-2.72 |
| 3 |
Campbell, Tessa N (13)
|
VARAYS |
37.59 |
40.39 |
2.80 |
| 4 |
Williams, Morgan L (14)
|
VARAYS |
41.91 |
43.89 |
1.98 |
| 5 |
Wang, Helen L (14)
|
VARAYS |
44.96 |
44.36 |
-0.60 |
| 6 |
Swanson, Guinevere G (13)
|
VADC |
48.51 |
45.86 |
-2.65 |
| 7 |
Daniel, Isabella M (13)
|
VARAYS |
44.16 |
46.72 |
2.56 |
| 8 |
Doppenberg, Isabel V (14)
|
VADC |
45.51 |
47.37 |
1.86 |
| 9 |
Riddle, Grace E (14)
|
VANOVA |
|
48.49 |
- |
| 10 |
Schueler, Gwyneth R (13)
|
VARAYS |
53.82 |
49.49 |
-4.33 |
| 11 |
Hinson, Jessica M (13)
|
VARAYS |
55.40 |
50.31 |
-5.09 |
| 12 |
McDowell, Cornelia M (13)
|
VADC |
|
52.35 |
- |
| 13 |
Singleton, Emily G (13)
|
VABASS |
1:07.73 |
1:01.35 |
-6.38 |
|
Zorn, Caroline A (13)
|
VANOVA |
1:01.12 |
NS |
- |
|
Hall, Cherish F (13)
|
VARAYS |
48.26 |
NS |
- |
|
Stephens, Lucy M (13)
|
VARAYS |
49.73 |
NS |
- |