| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bush, Meredith R (14)
|
NCMSA |
4:47.73 |
4:56.73 |
9.00 |
| 2 |
Eaton, Emily T (14)
|
NCMSA |
5:20.01 |
5:01.63 |
-18.38 |
| 3 |
Fender, Lillian K (13)
|
NCMHAC |
5:13.93 |
5:10.08 |
-3.85 |
| 4 |
Hollmeyer, Abigail L (14)
|
NCMSA |
4:54.61 |
5:12.03 |
17.42 |
| 5 |
Hendershot, Madelyn M (13)
|
NCSMAC |
5:16.27 |
5:14.78 |
-1.49 |
| 6 |
Wenhame, Rosalee T (13)
|
SEACAC |
5:14.37 |
5:15.76 |
1.39 |
| 7 |
Diaz, Sofia K (13)
|
NCMSA |
5:40.99 |
5:25.18 |
-15.81 |
| 8 |
Bonomo, Caroline L (14)
|
NCSMAC |
5:23.32 |
5:31.78 |
8.46 |
| 9 |
Steger, Natalie N (13)
|
SEACAC |
6:02.45 |
5:54.20 |
-8.25 |
|
Patterson, Lauren E (13)
|
NCUN |
4:55.84 |
4:58.60 |
2.76 |
|
Bozza, Natalie R (14)
|
NCMSA |
5:05.43 |
5:02.18 |
-3.25 |