| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Patterson, Lauren E (13)
|
NCUN |
2:29.24 |
2:35.79 |
6.55 |
| 2 |
Diaz, Sofia K (13)
|
NCMSA |
2:50.22 |
2:43.98 |
-6.24 |
| 3 |
Zollinger, Charlie N (13)
|
NCMSA |
2:56.43 |
2:50.42 |
-6.01 |
| 4 |
Brewster, Kylie A (14)
|
NCMSA |
3:01.77 |
2:51.46 |
-10.31 |
| 5 |
Hodge, Amelia R (13)
|
SEACAC |
2:50.09 |
2:52.81 |
2.72 |
| 6 |
Patrick, McKenna L (13)
|
NCMSA |
3:08.10 |
2:52.85 |
-15.25 |
| 7 |
Bonomo, Caroline L (14)
|
NCSMAC |
2:55.89 |
2:56.02 |
0.13 |
| 8 |
Kelley, Aubrey L (13)
|
NCMSA |
3:01.26 |
2:58.70 |
-2.56 |
| 9 |
Cushman, Mia G (13)
|
NCUN |
2:55.01 |
3:03.32 |
8.31 |
| 10 |
James, Abigail G (14)
|
NCMSA |
3:07.69 |
3:04.64 |
-3.05 |
| 11 |
Thompson, Jackson K (14)
|
NCMSA |
3:06.04 |
3:05.49 |
-0.55 |
| 12 |
Brentzel, Olivia M (13)
|
NCMSA |
|
3:05.54 |
- |
| 13 |
Martin, Sophie A (13)
|
NCMSA |
3:22.91 |
3:09.32 |
-13.59 |
| 14 |
Steger, Natalie N (13)
|
SEACAC |
|
3:10.55 |
- |
| 15 |
Swain, Stella B (13)
|
NCMSA |
|
3:11.47 |
- |
| 16 |
Christenson, Emery E (13)
|
NCMSA |
3:12.11 |
3:16.89 |
4.78 |
| 17 |
Clark, Harper A (14)
|
NCSMAC |
3:18.90 |
3:18.56 |
-0.34 |
| 18 |
Heckman, Julianna M (13)
|
NCMSA |
|
3:26.36 |
- |
| 19 |
Trimakas, Kate B (13)
|
NCMSA |
|
3:52.49 |
- |