| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Hsu, Kendrick Z (8)
|
AMPEAQ |
41.19 |
42.81 |
1.62 |
| 3 |
Kling, Charles P (8)
|
AMSVSC |
40.87 |
43.96 |
3.09 |
| 4 |
Ott, Benjamin J (8)
|
AMPEAQ |
44.71 |
44.81 |
0.10 |
| 5 |
Grajeda, Patricio A (8)
|
AMWAAC |
41.39 |
45.68 |
4.29 |
| 6 |
Hagenstein, Carson B (8)
|
AMWAAC |
46.44 |
50.20 |
3.76 |
| 7 |
Butler, Brier W (8)
|
AMWAAC |
55.23 |
56.01 |
0.78 |
| 8 |
Zhao, Jayden W (6)
|
AMPEAQ |
59.64 |
1:01.45 |
1.81 |
| 9 |
Robertson, Derek J (8)
|
AMWAAC |
1:02.21 |
1:02.27 |
0.06 |
| 10 |
Chandiran, Sai Suriya X (8)
|
AMPEAQ |
59.83 |
1:05.59 |
5.76 |
| 11 |
Carney, August D (8)
|
AMPEAQ |
1:08.13 |
1:29.42 |
21.29 |
|
Bise, Bennett C (6)
|
AMPEAQ |
1:31.35 |
NS |
- |
|
Ott, William F (8)
|
AMPEAQ |
49.02 |
NS |
- |
|
Khattab, Rayan (8)
|
AMSFAC |
57.87 |
NS |
- |
|
Gopalakrishnan, Gautam (7)
|
AMWAAC |
1:00.63 |
NS |
- |