| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cupid, Benjamin E (9)
|
FGPST |
44.40 |
38.20 |
-6.20 |
| 2 |
Cavani, Emiliano (10)
|
FGSOFL |
43.05 |
42.45 |
-0.60 |
| 3 |
McVeigh, Dylan M (9)
|
FGSOFL |
43.28 |
43.19 |
-0.09 |
| 4 |
Spiller, Jonah D (9)
|
FGHAT |
44.25 |
43.45 |
-0.80 |
| 5 |
Corrales, Francesco (9)
|
FGSFTL |
46.04 |
44.27 |
-1.77 |
| 6 |
Christensen, Preston R (9)
|
FGSOFL |
45.25 |
45.38 |
0.13 |
| 7 |
Nichols, Christian (10)
|
FGTSA |
45.74 |
46.21 |
0.47 |
| 8 |
Forgione, Luigi (10)
|
FGAZFL |
48.24 |
46.55 |
-1.69 |
| 9 |
Valdiviezo, David E (9)
|
FGSF |
50.42 |
47.30 |
-3.12 |
| 10 |
Burgos, Santiago (9)
|
FGAZFL |
1:02.00 |
47.92 |
-14.08 |
| 11 |
Carrera, Zacchaeus J (9)
|
FGUN-0 |
48.58 |
48.95 |
0.37 |
| 12 |
Agashe, Atharv H (9)
|
FGSOFL |
|
49.22 |
- |
| 13 |
DeSouza, Joshua (10)
|
FGCSSC |
46.95 |
50.32 |
3.37 |
| 14 |
Brinkmann, Pascal D (9)
|
FGHAT |
50.06 |
50.45 |
0.39 |
| 15 |
Dogan, Alessandro (9)
|
FGSFTL |
57.69 |
52.95 |
-4.74 |
| 16 |
Paruchuru, Sridhyan (10)
|
FGCSSC |
50.80 |
53.11 |
2.31 |
| 17 |
Carey, Dylan (9)
|
FGSFTL |
49.89 |
54.41 |
4.52 |
| 18 |
Carlucci, Sean M (10)
|
FGCSSC |
55.62 |
55.64 |
0.02 |
| 19 |
Applegate, Dominik R (9)
|
FGSUN |
53.88 |
58.16 |
4.28 |
| 20 |
Neves, Landon A (9)
|
FGCCPR |
|
1:01.20 |
- |
| 21 |
Evans, Jonathan C (9)
|
FGTSA |
1:07.40 |
1:06.64 |
-0.76 |
|
Lee, Jacob T (9)
|
FGSOFL |
|
DQ |
- |
|
Rodriguez-Cedeno, Johzua (10)
|
FGTSA |
|
DQ |
- |
|
Kozman, Michael (10)
|
FGSFTL |
1:07.68 |
NS |
- |