| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smit, Olivia K (8)
|
MDASC |
45.33 |
36.22 |
-9.11 |
| 2 |
Xu, Sophia D (8)
|
MDEST |
39.61 |
36.61 |
-3.00 |
| 3 |
Wu, Sunny (8)
|
MDCAA |
42.49 |
37.94 |
-4.55 |
| 4 |
Sites, Madison J (8)
|
MDHOCO |
47.71 |
40.76 |
-6.95 |
| 5 |
Matanoski, Genevieve A (8)
|
MDEST |
45.57 |
41.99 |
-3.58 |
| 6 |
Bickel, Kelly E (8)
|
MDHOCO |
42.34 |
42.08 |
-0.26 |
| 7 |
Ohtani, Mizuki (8)
|
MDCAA |
|
43.01 |
- |
| 8 |
Reese, Micah J (8)
|
MDASC |
51.01 |
44.04 |
-6.97 |
| 9 |
Long, Megan O (8)
|
MDCAA |
52.48 |
44.20 |
-8.28 |
| 10 |
Swope, Lily M (8)
|
MDASC |
44.84 |
44.48 |
-0.36 |
| 11 |
Boarman, Ann M (8)
|
MDEST |
|
44.86 |
- |
| 12 |
Fitzsimmons, Christina M (8)
|
MDCAA |
|
46.00 |
- |
| 13 |
Shinskie, Taylor L (8)
|
MDHOCO |
|
46.29 |
- |
| 14 |
Otero, Marisa L (8)
|
MDCAA |
43.75 |
46.77 |
3.02 |
| 15 |
Rothrock, Abigale P (8)
|
MDCAA |
|
47.09 |
- |
| 16 |
Workman, Kate F (8)
|
MDMAS |
|
47.73 |
- |
| 17 |
Chandler, Campbell F (8)
|
MDMAS |
|
48.55 |
- |
| 18 |
Madden, Alexis C (8)
|
MDASC |
51.62 |
49.31 |
-2.31 |
| 19 |
Vijay, Ananya (8)
|
MDCAA |
51.02 |
50.00 |
-1.02 |
| 20 |
Akhtarkhavari, Arshin (8)
|
MDCAA |
|
50.50 |
- |
| 21 |
Agrawal, Sofya L (8)
|
MDCAA |
|
50.96 |
- |
| 22 |
Essa, Osob B (8)
|
MDASC |
1:01.02 |
51.73 |
-9.29 |
| 23 |
Wang, Amanda (8)
|
MDHOCO |
56.47 |
52.29 |
-4.18 |
| 24 |
DeLessio, Sarena G (8)
|
MDHOCO |
|
52.69 |
- |
| 25 |
Melchionni, Elizabeth M (8)
|
MDMAS |
|
53.31 |
- |
| 26 |
Wang, Anna C (8)
|
MDEST |
58.58 |
53.51 |
-5.07 |
| 27 |
Rizas, Anastasia M (8)
|
MDCAA |
|
53.60 |
- |
| 28 |
Hutchins, Rachel M (8)
|
MDCAA |
48.53 |
54.76 |
6.23 |
| 29 |
Yusubov, Diana L (8)
|
MDEST |
59.35 |
56.07 |
-3.28 |
| 30 |
Dalton, Evelyn E (8)
|
MDCAA |
|
56.14 |
- |
|
Gioffreda, Megan G (8)
|
MDMAS |
|
NS |
- |