| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smit, Olivia K (8)
|
MDASC |
43.83 |
42.53 |
-1.30 |
| 2 |
Wu, Sunny (8)
|
MDCAA |
44.46 |
42.70 |
-1.76 |
| 3 |
Xu, Sophia D (8)
|
MDEST |
44.58 |
44.86 |
0.28 |
| 4 |
Matanoski, Genevieve A (8)
|
MDEST |
|
51.32 |
- |
| 5 |
Bickel, Kelly E (8)
|
MDHOCO |
51.39 |
53.16 |
1.77 |
| 6 |
Fitzsimmons, Christina M (8)
|
MDCAA |
|
58.31 |
- |
| 7 |
Long, Megan O (8)
|
MDCAA |
|
1:00.32 |
- |
| 8 |
Rothrock, Abigale P (8)
|
MDCAA |
|
1:01.21 |
- |
| 9 |
Yusubov, Diana L (8)
|
MDEST |
1:01.46 |
1:03.22 |
1.76 |
|
Vijay, Ananya (8)
|
MDCAA |
56.52 |
DQ |
- |
|
Boarman, Ann M (8)
|
MDEST |
|
DQ |
- |
|
Wang, Anna C (8)
|
MDEST |
|
DQ |
- |