| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wong, Annika K (10)
|
NTCOPS |
39.59 |
36.17 |
-3.42 |
| 2 |
Lindahl, Katherine M (10)
|
NTCOPS |
38.40 |
36.19 |
-2.21 |
| 3 |
Groff, Emily M (10)
|
NTCOPS |
37.17 |
36.94 |
-0.23 |
| 4 |
Sears, Ainslee K (8)
|
NTCOPS |
37.06 |
37.01 |
-0.05 |
| 5 |
Escutia Castaneda, Ariadna (10)
|
NTCOPS |
|
37.14 |
- |
| 6 |
Fitzgerald, Taryn S (9)
|
NTCOPS |
42.18 |
37.41 |
-4.77 |
| 7 |
Wall, Emily D (7)
|
NTCOPS |
40.89 |
38.17 |
-2.72 |
| 8 |
Shih, Tina K (10)
|
NTCOPS |
|
38.20 |
- |
| 9 |
Tarnavsky, Mila B (10)
|
NTESP |
|
38.27 |
- |
| 10 |
Bouressa, Olivia N (10)
|
NTCOPS |
45.13 |
41.09 |
-4.04 |
| 11 |
Greiner, Madelynn M (8)
|
NTCOPS |
41.15 |
41.15 |
0.00 |
| 12 |
Wesneski, Abigail R (10)
|
NTCOPS |
|
42.22 |
- |
| 13 |
Peck, Zoe L (9)
|
NTESP |
43.80 |
42.84 |
-0.96 |
| 14 |
Dalton, Cayden C (10)
|
NTCOPS |
|
44.07 |
- |
| 15 |
Richardson, Maris O (7)
|
NTESP |
|
57.88 |
- |