| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Groff, Emily M (10)
|
NTCOPS |
50.74 |
47.13 |
-3.61 |
| 2 |
Lindahl, Katherine M (10)
|
NTCOPS |
50.29 |
48.41 |
-1.88 |
| 3 |
Shih, Tina K (10)
|
NTCOPS |
|
50.02 |
- |
| 4 |
Sears, Ainslee K (8)
|
NTCOPS |
52.01 |
50.78 |
-1.23 |
| 5 |
Peck, Zoe L (9)
|
NTESP |
57.78 |
51.18 |
-6.60 |
| 6 |
Fitzgerald, Taryn S (9)
|
NTCOPS |
55.14 |
51.36 |
-3.78 |
| 7 |
Escutia Castaneda, Ariadna (10)
|
NTCOPS |
|
51.93 |
- |
| 8 |
Wong, Annika K (10)
|
NTCOPS |
48.26 |
52.51 |
4.25 |
| 9 |
Tarnavsky, Mila B (10)
|
NTESP |
|
54.25 |
- |
| 10 |
Wesneski, Abigail R (10)
|
NTCOPS |
58.59 |
54.48 |
-4.11 |
| 11 |
Wall, Emily D (7)
|
NTCOPS |
57.22 |
58.15 |
0.93 |
|
Bouressa, Olivia N (10)
|
NTCOPS |
56.18 |
DQ |
- |
|
Dalton, Cayden C (10)
|
NTCOPS |
|
DQ |
- |
|
Greiner, Madelynn M (8)
|
NTCOPS |
53.09 |
DQ |
- |