| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bowling, Kiera B (13)
|
NTMTRO |
1:02.12 |
59.45 |
-2.67 |
| 2 |
Morton, Madelyn J (14)
|
NTCOPS |
1:00.49 |
1:01.79 |
1.30 |
| 3 |
Morales, Michele (13)
|
NTCOR |
1:01.66 |
1:02.05 |
0.39 |
| 4 |
Sizemore, Danica X (13)
|
NTMTRO |
1:02.62 |
1:02.95 |
0.33 |
| 5 |
Liles, Natalie T (14)
|
NTMTRO |
1:09.64 |
1:04.80 |
-4.84 |
| 6 |
Dooley, Ellen J (13)
|
NTCOPS |
1:05.28 |
1:05.14 |
-0.14 |
| 7 |
Rallapalli, Adithi L (14)
|
NTCOR |
1:03.74 |
1:05.75 |
2.01 |
| 8 |
Schaefer, Grace A (13)
|
NTCOPS |
1:05.95 |
1:10.46 |
4.51 |
| 9 |
Fusco, Siena C (13)
|
NTCOPS |
1:12.02 |
1:16.60 |
4.58 |
| 10 |
Nukala, Aditi L (13)
|
NTCOPS |
1:16.25 |
1:16.94 |
0.69 |
| 11 |
Reddy, Anvi V (13)
|
NTCOPS |
1:18.48 |
1:19.82 |
1.34 |
| 12 |
Spencer, Eden K (13)
|
NTCOR |
1:26.11 |
1:25.36 |
-0.75 |
|
Unterstein, Elliyah R (14)
|
NTCOPS |
1:03.68 |
NS |
- |
|
Zhu, Annie Q (13)
|
NTCOPS |
1:18.08 |
NS |
- |
|
Waidler, Ruby M (13)
|
NTCOR |
1:09.06 |
NS |
- |
|
George, Taylor K (13)
|
NTMTRO |
1:02.22 |
NS |
- |
|
Ciske, Grace A (14)
|
NTCOPS |
1:02.49 |
NS |
- |