| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adams, Jacob A (14)
|
NTCOR |
25.83 |
25.71 |
-0.12 |
| 2 |
Wang, Daniel H (14)
|
NTMTRO |
26.84 |
25.99 |
-0.85 |
| 3 |
McWard, Eli W (13)
|
NTMTRO |
26.74 |
26.36 |
-0.38 |
| 4 |
Yu, Victor K (14)
|
NTMTRO |
26.59 |
26.41 |
-0.18 |
| 5 |
Vishnubhotla, Arihant S (13)
|
NTUNAT |
27.17 |
26.44 |
-0.73 |
| 6 |
Ni, Linden E (13)
|
NTCOPS |
26.23 |
26.45 |
0.22 |
| 7 |
Torres, Abiel R (13)
|
NTCOR |
26.49 |
26.65 |
0.16 |
| 8 |
Kang, Christian K (13)
|
NTMTRO |
29.52 |
27.55 |
-1.97 |
| 9 |
Kwon, Jisoo (13)
|
NTMTRO |
|
27.77 |
- |
| 10 |
Hansen, Benjamin V (14)
|
NTMTRO |
28.30 |
28.37 |
0.07 |
| 11 |
Elliott, Luke E (13)
|
NTCOPS |
28.10 |
28.45 |
0.35 |
| 12 |
Mele, David M (13)
|
NTCOPS |
28.64 |
28.89 |
0.25 |
| 13 |
Alguire, Landon C (13)
|
NTMTRO |
31.87 |
30.74 |
-1.13 |
| 14 |
Parikh, Shayan A (13)
|
NTCOPS |
31.02 |
30.92 |
-0.10 |
| 15 |
Stone, Austin K (14)
|
NTMTRO |
30.75 |
30.97 |
0.22 |
| 16 |
Trivedi, Arjun H (13)
|
NTMTRO |
33.12 |
31.54 |
-1.58 |
| 17 |
Bankapur, Amogh A (14)
|
NTMTRO |
38.38 |
32.56 |
-5.82 |
| 18 |
Bittner, Evan C (13)
|
NTMTRO |
32.26 |
32.62 |
0.36 |
| 19 |
Kapoor, Ayush (14)
|
NTCOPS |
32.67 |
32.84 |
0.17 |
| 20 |
Anderson, Nicholas W (13)
|
NTMTRO |
37.70 |
33.03 |
-4.67 |
| 21 |
Anderson, Connor A (14)
|
NTSTAR |
|
33.82 |
- |
| 22 |
Tata, Vishnu S (13)
|
NTMTRO |
|
38.23 |
- |
|
Tesdall, Lyle T (14)
|
NTCOPS |
34.96 |
NS |
- |
|
Tang, Kevin M (14)
|
NTSTAR |
47.74 |
NS |
- |