| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gotham, Madison E (15)
|
NTMTRO |
1:02.61 |
1:04.04 |
1.43 |
| 2 |
Harless, Madison E (16)
|
NTMTRO |
1:14.45 |
1:05.53 |
-8.92 |
| 3 |
Alberts, Payton F (16)
|
NTMTRO |
1:06.89 |
1:06.59 |
-0.30 |
| 4 |
Huang, Katherine (16)
|
NTDM |
1:03.63 |
1:06.99 |
3.36 |
| 5 |
Shimer, Meghan E (17)
|
NTSTAR |
1:02.30 |
1:08.34 |
6.04 |
| 6 |
Deitemeyer, Abby M (15)
|
NTMTRO |
1:03.11 |
1:08.50 |
5.39 |
| 7 |
Larson, Jenna G (17)
|
NTUN |
1:04.61 |
1:08.96 |
4.35 |
| 8 |
Costantini, Amelia L (15)
|
NTSTAR |
1:08.71 |
1:09.76 |
1.05 |
| 9 |
Noone, Chloe L (15)
|
NTMTRO |
1:07.22 |
1:10.25 |
3.03 |
| 10 |
Francis, Sydney C (15)
|
NTSTAR |
1:14.00 |
1:17.93 |
3.93 |
|
Stidham, Catherine E (16)
|
NTDM |
1:13.18 |
NS |
- |
|
Eidman, Natalie M (15)
|
NTLTSD |
1:07.83 |
NS |
- |