| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zaryski, Eleanor A (10)
|
NICSCI |
46.38 |
46.48 |
0.10 |
| 2 |
Stojanovski, Nadia E (10)
|
NICSCI |
48.30 |
48.20 |
-0.10 |
| 3 |
Sparks, Julia M (10)
|
NICSCI |
50.23 |
49.69 |
-0.54 |
| 4 |
Krueger, Claire J (9)
|
NIJETS |
53.27 |
49.83 |
-3.44 |
| 5 |
Fitzgerald, Nora M (9)
|
NICSCI |
53.12 |
53.58 |
0.46 |
| 6 |
Tarolli, Evangelina G (9)
|
NICSCI |
54.64 |
54.44 |
-0.20 |
| 7 |
Haney, Corinne R (8)
|
ADTBS |
58.89 |
57.74 |
-1.15 |
| 8 |
Nicol, Mikayla E (10)
|
ADTBS |
59.54 |
58.92 |
-0.62 |
| 9 |
Law, Brenna M (9)
|
ADTBS |
59.93 |
1:00.67 |
0.74 |
| 10 |
Bohli, Camille N (9)
|
NICSCI |
|
1:03.63 |
- |
| 11 |
Sagneri, Julianna M (9)
|
NICSCI |
|
1:04.25 |
- |
| 12 |
Earl, Sofia R (9)
|
NICSCI |
|
1:04.35 |
- |
| 13 |
Francescotti, Maria G (9)
|
NIJETS |
1:02.86 |
1:05.30 |
2.44 |
| 14 |
Starowicz, Avery G (9)
|
NICSCI |
|
1:06.69 |
- |
| 15 |
Hann, Rachel S (9)
|
NICSCI |
1:07.20 |
1:06.78 |
-0.42 |
| 16 |
Clabaugh, Skyler B (9)
|
NICSCI |
|
1:07.77 |
- |
| 17 |
Stewart, Bailey F (8)
|
NIBSSC |
|
1:11.19 |
- |
| 18 |
Halstead, Brooke L (10)
|
NIJETS |
1:14.25 |
1:19.74 |
5.49 |
|
Shortslef, Allison C (10)
|
NICSCI |
|
DQ |
- |
|
Sparks, Olivia M (9)
|
NICSCI |
|
DQ |
- |
|
Price, Ashlynn M (9)
|
NICSCI |
|
DQ |
- |
|
Blount, Ella (10)
|
NISKAN |
|
DQ |
- |
|
Neuser, Skylar R (10)
|
NICSCI |
|
NS |
- |