| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zaryski, Eleanor A (11)
|
NICSCI |
41.84 |
41.89 |
0.05 |
| 2 |
Bohman, Paige D (12)
|
NIBSSC |
42.41 |
42.41 |
0.00 |
| 3 |
Gittschau, Angelina M (12)
|
NICSCI |
47.81 |
42.71 |
-5.10 |
| 4 |
Sparks, Julia M (11)
|
NICSCI |
37.84 |
42.91 |
5.07 |
| 5 |
Stojanovski, Nadia E (11)
|
NICSCI |
43.18 |
43.85 |
0.67 |
| 6 |
Gluz, Elizabeth A (12)
|
NICSCI |
50.54 |
48.08 |
-2.46 |
| 7 |
Swenton, Haley R (12)
|
NINAYE |
50.87 |
52.00 |
1.13 |
| 8 |
Dykes, Ayva R (12)
|
NICSCI |
1:08.75 |
52.91 |
-15.84 |
| 9 |
DiLiberto, Jenna M (11)
|
NINAYE |
51.78 |
53.78 |
2.00 |
| 10 |
Soprano, Sophia (11)
|
NISKAN |
1:04.07 |
54.94 |
-9.13 |
| 11 |
Walker, Delaney J (12)
|
NICSCI |
|
55.88 |
- |
| 12 |
Latessa, Rose G (12)
|
NICSCI |
51.94 |
56.56 |
4.62 |
| 13 |
Neuser, Skylar R (11)
|
NICSCI |
1:03.11 |
1:04.71 |
1.60 |
|
Petralia, Bella C (11)
|
NINAYE |
1:10.00 |
DQ |
- |
|
Hlad, Emmilee C (12)
|
ADTBS |
|
DQ |
- |
|
Tully, Laura A (11)
|
NICSCI |
|
NS |
- |