| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tarolli, Vincent M (15)
|
NICSCI |
52.38 |
52.50 |
0.12 |
| 2 |
Scharoun, Keegan N (15)
|
NICSCI |
54.39 |
55.47 |
1.08 |
| 3 |
Olmsted, Sam T (16)
|
NISKAN |
54.04 |
56.22 |
2.18 |
| 4 |
Barrington, Matthew W (15)
|
NICSCI |
1:04.48 |
59.41 |
-5.07 |
| 5 |
Drotar, Zach (13)
|
NISKAN |
1:01.20 |
1:02.52 |
1.32 |
| 6 |
Denkenberger, Quinten M (13)
|
NIOLSC |
1:02.87 |
1:07.28 |
4.41 |
| 7 |
Sledziona, Jaco (14)
|
NIOLSC |
1:17.28 |
1:16.47 |
-0.81 |
| 8 |
Tsymbala, Denys (12)
|
NICSCI |
|
1:25.31 |
- |
| 9 |
Milliman, Lathan (9)
|
NIWGSC |
1:43.81 |
1:37.09 |
-6.72 |
| 10 |
Krul, Landon C (10)
|
NIWGSC |
1:56.31 |
1:46.00 |
-10.31 |
|
Petralia, Logan J (16)
|
NINAYE |
57.90 |
NS |
- |
|
Thomas, Joshua D (17)
|
NIOLSC |
50.02 |
NS |
- |
|
Wells, Ben A (15)
|
NISKAN |
54.93 |
NS |
- |