| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roopnarine, Josha A (12)
|
ADBENN |
37.13 |
36.77 |
-0.36 |
| 2 |
Hanna, Nathan C (12)
|
ADNHA |
37.11 |
36.94 |
-0.17 |
| 3 |
Barlow, Nicholas M (12)
|
ADCANA |
38.74 |
37.50 |
-1.24 |
| 4 |
Morgan, Finn E (12)
|
ADCLRK |
38.09 |
37.78 |
-0.31 |
| 5 |
Holmes, Sheldon H (12)
|
ADNHA |
39.83 |
37.89 |
-1.94 |
| 6 |
Leanza, Christopher M (12)
|
ADSCHE |
38.88 |
39.72 |
0.84 |
| 7 |
McNair, John P (11)
|
ADCLIN |
38.47 |
39.80 |
1.33 |
| 8 |
Wilkosz, Matthew M (11)
|
ADCLIN |
39.83 |
41.11 |
1.28 |
| 9 |
Tresnjo, Arman (12)
|
ADNHA |
44.96 |
41.46 |
-3.50 |
| 10 |
Hovak, Aiden P (12)
|
ADNHA |
43.08 |
42.40 |
-0.68 |
| 11 |
Forrest, Parker G (11)
|
ADCANA |
43.73 |
42.67 |
-1.06 |
| 12 |
Nuccio, Dominic S (11)
|
ADBENN |
42.68 |
42.94 |
0.26 |
| 13 |
Robinson, Samuel J (12)
|
ADCANA |
45.03 |
43.20 |
-1.83 |
| 14 |
Thibault, Cain W (11)
|
ADCLIN |
44.44 |
43.54 |
-0.90 |
| 15 |
Kasper, Noah K (11)
|
ADSCHE |
49.19 |
43.77 |
-5.42 |
| 16 |
Raymond, John L (12)
|
ADCLRK |
48.99 |
45.11 |
-3.88 |
| 17 |
Conrad, Gavin A (11)
|
ADADDI |
45.62 |
45.45 |
-0.17 |
| 18 |
Loghin, Victor G (12)
|
ADSCHE |
51.41 |
46.40 |
-5.01 |
| 19 |
Harding, Daren C (11)
|
ADCANA |
46.15 |
47.55 |
1.40 |
| 20 |
Walsh, Evan T (11)
|
ADSCHE |
44.53 |
47.89 |
3.36 |
| 21 |
Xu, Richard Z (12)
|
ADNHA |
|
49.44 |
- |
| 22 |
Wilson, Luke M (12)
|
ADNHA |
52.32 |
50.63 |
-1.69 |
| 23 |
Brych, Niholas M (11)
|
ADCLIN |
52.67 |
50.94 |
-1.73 |
| 24 |
Bixby, Jameson S (11)
|
ADSVP |
53.37 |
55.57 |
2.20 |
| 25 |
Hasenauer, Ethan M (11)
|
ADNHA |
|
58.69 |
- |
| 26 |
Yastrzemski, Aleksander C (11)
|
ADCLRK |
1:00.48 |
59.72 |
-0.76 |
|
Herringshaw, Jacob R (11)
|
ADCANA |
1:51.93 |
DQ |
- |
|
Wang, Jake H (12)
|
ADSCHE |
40.24 |
NS |
- |