| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCarthy, Ella I (12)
|
ADNHA |
47.51 |
38.88 |
-8.63 |
| 2 |
Walker, Eleanor A (12)
|
ADCLRK |
43.68 |
43.57 |
-0.11 |
| 3 |
Lytel, Eve O (12)
|
ADCLRK |
46.36 |
45.94 |
-0.42 |
| 4 |
Lebert, Hannah M (11)
|
ADBENN |
46.30 |
47.08 |
0.78 |
| 5 |
Heiner, Elizabeth A (12)
|
ADNHA |
51.58 |
47.12 |
-4.46 |
| 6 |
Jaros, Elaina N (11)
|
ADNHA |
48.61 |
49.03 |
0.42 |
| 7 |
Applebaum, Aaliyah R (12)
|
ADCANA |
50.65 |
49.98 |
-0.67 |
| 8 |
Dugan, Chrisgianna R (12)
|
ADSCHE |
|
51.05 |
- |
| 9 |
Kelly, Teagan M (11)
|
ADCANA |
54.91 |
52.33 |
-2.58 |
| 10 |
Trafton, Abbie C (11)
|
ADBENN |
58.51 |
54.86 |
-3.65 |
|
Fernandez-Turo, Rocio A (12)
|
ADNHA |
56.03 |
DQ |
- |
|
Budhu, Sonia M (12)
|
ADNHA |
|
DQ |
- |
|
Alcuri, Julia M (12)
|
ADNHA |
|
DQ |
- |
|
Jenkins, Clara C (11)
|
ADNHA |
|
DQ |
- |
|
Luley, Mya E (12)
|
ADNHA |
|
DQ |
- |
|
Patel, Avni B (11)
|
ADCLRK |
1:07.70 |
DQ |
- |
|
Bong, Laila G (11)
|
ADCLRK |
|
DQ |
- |