| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kelly, Teagan M (12)
|
ADCANA |
32.76 |
32.85 |
0.09 |
| 2 |
Carey, Schuyler C (12)
|
ADNHA |
33.51 |
33.53 |
0.02 |
| 3 |
Massoud, Terrianne S (11)
|
ADNHA |
33.83 |
34.26 |
0.43 |
| 4 |
Lebert, Hannah M (12)
|
ADBENN |
37.81 |
35.83 |
-1.98 |
| 5 |
Willey, Lyla G (12)
|
ADBENN |
45.03 |
38.01 |
-7.02 |
| 6 |
Obernesser, Natalie C (11)
|
ADNHA |
38.82 |
38.39 |
-0.43 |
| 7 |
Fahey, Reagan (12)
|
ADBENN |
|
39.52 |
- |
| 8 |
Bong, Violet F (11)
|
ADCLRK |
40.60 |
39.74 |
-0.86 |
| 9 |
Kenyon, Hayden K (12)
|
ADCLRK |
57.01 |
42.14 |
-14.87 |
| 10 |
Wallace, Mora N (11)
|
ADNHA |
48.49 |
42.78 |
-5.71 |
| 11 |
Carpenter, Isabella G (11)
|
ADNHA |
45.88 |
46.77 |
0.89 |
| 12 |
Merrell, Julia K (11)
|
ADNHA |
41.72 |
47.31 |
5.59 |
| 13 |
Phatak, Nabhaa U (12)
|
ADNHA |
|
53.13 |
- |
| 14 |
Patsos, Yara A (12)
|
ADCANA |
58.80 |
57.93 |
-0.87 |