| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rio, Willa K (8)
|
ADCLIN |
51.38 |
43.63 |
-7.75 |
| 2 |
Walker, Eleanor A (8)
|
ADCLRK |
48.81 |
43.71 |
-5.10 |
| 3 |
Yager, Myah Y (8)
|
ADCANA |
51.69 |
46.16 |
-5.53 |
| 4 |
Panetta, Skylar J (7)
|
ADSCHE |
48.82 |
48.95 |
0.13 |
| 5 |
Applebaum, Aaliyah R (8)
|
ADCANA |
52.36 |
50.59 |
-1.77 |
| 6 |
McAllister, Riley A (7)
|
ADJJSC |
53.81 |
51.14 |
-2.67 |
| 7 |
Dame, Meredith A (7)
|
ADBENN |
55.34 |
54.13 |
-1.21 |
| 8 |
Spenello-Carlton, Giavanna E (7)
|
ADSVP |
1:15.00 |
54.15 |
-20.85 |
| 9 |
Gollhofer, Madeline C (7)
|
ADCLRK |
58.52 |
57.47 |
-1.05 |
| 10 |
Trafton, Abbie C (7)
|
ADBENN |
|
58.27 |
- |
| 11 |
Adam, Sophia M (8)
|
ADCLRK |
59.27 |
58.28 |
-0.99 |
| 12 |
Strickler, Carolyn M (7)
|
ADSCHE |
|
58.72 |
- |
| 13 |
Klein, Carter A (8)
|
ADSCHE |
56.59 |
59.02 |
2.43 |
| 14 |
Donato, Alexandra C (8)
|
ADSCHE |
58.36 |
59.43 |
1.07 |
| 15 |
Dugan, Chrisgianna R (8)
|
ADSCHE |
1:20.77 |
59.90 |
-20.87 |
| 16 |
Reardon, Caroline E (7)
|
ADSCHE |
|
1:01.02 |
- |
| 17 |
Nuccio, Faydra M (7)
|
ADBENN |
1:14.93 |
1:02.67 |
-12.26 |
| 18 |
Misiewicz, Noah E (8)
|
ADCLRK |
|
1:04.05 |
- |
| 19 |
Attia, Laila (7)
|
ADSCHE |
|
1:05.77 |
- |
| 20 |
Yastrzemski, Sofia Jane J (8)
|
ADCLRK |
|
1:06.15 |
- |
| 21 |
Geckler, Elle K (7)
|
ADSCHE |
|
2:05.66 |
- |