| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Polite-Koulouris, Kassandra (9)
|
ADCANA |
46.58 |
49.54 |
2.96 |
| 2 |
Loiacano, Michaela F (10)
|
ADNHA |
|
50.01 |
- |
| 3 |
Coffay, Karina M (9)
|
ADNHA |
|
50.07 |
- |
| 4 |
Merrell, Charlotte R (10)
|
ADNHA |
50.90 |
50.66 |
-0.24 |
| 5 |
Douglass, Laila M (10)
|
ADCANA |
50.99 |
50.86 |
-0.13 |
| 6 |
Mandlik, Deeti S (10)
|
ADNHA |
|
52.39 |
- |
| 7 |
Foster, Meara E (9)
|
ADCLRK |
55.02 |
53.72 |
-1.30 |
| 8 |
Foster, Rosalie G (9)
|
ADCLRK |
53.00 |
54.40 |
1.40 |
| 9 |
Matt, Keira M (10)
|
ADNHA |
53.58 |
54.92 |
1.34 |
| 10 |
White, Selena A (10)
|
ADNHA |
1:02.82 |
56.71 |
-6.11 |
| 11 |
Myers, Jane A (10)
|
ADCLIN |
|
58.12 |
- |
| 12 |
Carroll-Dorn, Lillian E (9)
|
ADNHA |
|
1:01.77 |
- |
| 13 |
Alden, Nyssa C (10)
|
ADCLRK |
1:03.27 |
1:02.72 |
-0.55 |
| 14 |
Fernandez-Turo, Jimena M (9)
|
ADNHA |
1:13.03 |
1:03.71 |
-9.32 |
| 15 |
Walsh, Summer R (10)
|
ADNHA |
1:09.02 |
1:03.86 |
-5.16 |
| 16 |
Betts, Luna C (8)
|
ADCANA |
|
1:03.87 |
- |
| 17 |
Brownell, Evelyn G (7)
|
ADBENN |
|
1:07.63 |
- |
|
Amos, Quinn L (9)
|
ADCLRK |
56.08 |
DQ |
- |