| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Phillips, Lily E (14)
|
VACA-Y |
25.48 |
26.09 |
0.61 |
| 2 |
Phillips, Rowena B (13)
|
VALAC |
26.47 |
27.39 |
0.92 |
| 3 |
Mahland, Kaelyn M (13)
|
VALAC |
27.32 |
28.14 |
0.82 |
| 4 |
Loura, Elaina R (13)
|
VACA-Y |
28.50 |
28.41 |
-0.09 |
| 5 |
Wayner, Sophia H (14)
|
VACA-Y |
29.46 |
29.41 |
-0.05 |
| 6 |
Manela, Joanna R (14)
|
PVPAC |
30.97 |
29.73 |
-1.24 |
| 7 |
Streit, Dorothy V (13)
|
VACA-Y |
28.70 |
29.87 |
1.17 |
| 8 |
Showalter, Tessa L (13)
|
VACA-Y |
30.42 |
30.12 |
-0.30 |
| 9 |
He, Sarina (14)
|
VACA-Y |
31.08 |
30.20 |
-0.88 |
| 10 |
Hendershot, Kelsi A (13)
|
PVPAC |
29.65 |
30.45 |
0.80 |
| 11 |
McDerment, Hannah G (14)
|
PVPAC |
31.97 |
31.88 |
-0.09 |
| 12 |
Cowperthwaite, Abigail M (13)
|
PVPAC |
33.09 |
33.81 |
0.72 |
| 13 |
Ferus, Viktoria (13)
|
PVPAC |
34.35 |
34.06 |
-0.29 |
| 14 |
Stawinski, Alexandra U (13)
|
PVPAC |
43.54 |
48.76 |
5.22 |