| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blair, Ella (14)
|
PNSVFY |
30.66 |
28.78 |
-1.88 |
| 2 |
Anderson, Brooke R (13)
|
PNDTY |
28.88 |
29.02 |
0.14 |
| 3 |
Wetherington, Emme A (13)
|
PNNSYS |
29.73 |
30.40 |
0.67 |
| 4 |
Kelsey, Torin (13)
|
PNSVFY |
32.24 |
31.58 |
-0.66 |
| 5 |
Fernandez, Gabrielle (13)
|
PNSVFY |
31.10 |
31.59 |
0.49 |
| 6 |
Fowler, Violet (14)
|
PNSVFY |
34.14 |
32.74 |
-1.40 |
| 7 |
Selsor, Sarah R (13)
|
PNSVFY |
36.00 |
32.81 |
-3.19 |
| 8 |
Chen, Mia (14)
|
PNNSYS |
33.35 |
33.34 |
-0.01 |
| 9 |
Broderhausen, Audrey (13)
|
PNDTY |
33.91 |
33.54 |
-0.37 |
| 10 |
Burrill, Lana (13)
|
PNWYD |
35.00 |
34.16 |
-0.84 |
| 11 |
Herrell, Simone (13)
|
PNNSYS |
34.52 |
34.40 |
-0.12 |
| 12 |
Obuon, Malaika (13)
|
MCY |
37.22 |
35.21 |
-2.01 |
| 13 |
Smitkin, Sabrina (14)
|
MCY |
36.45 |
36.21 |
-0.24 |
| 14 |
Schiebel, Sofia G (13)
|
PNNSYS |
34.83 |
36.24 |
1.41 |
| 15 |
Shrestha, Neeva (14)
|
MCY |
37.34 |
36.68 |
-0.66 |
| 16 |
Albright, Rachel (14)
|
MCY |
37.29 |
38.90 |
1.61 |
| 17 |
Kline, Madeline (13)
|
PNDTY |
42.35 |
39.78 |
-2.57 |
| 18 |
Nguyen, Mai (13)
|
MCY |
41.76 |
40.62 |
-1.14 |
|
Nguyen, Trinity (12)
|
PNNSYS |
31.19 |
NS |
- |
|
VanderWeyst, Heidi M (13)
|
PNNSYS |
33.90 |
NS |
- |
|
Allen, Charlotte C (12)
|
PNNSYS |
31.78 |
NS |
- |
|
Ye, Priscilla (13)
|
PNNSYS |
42.17 |
NS |
- |
|
Santangelo, Heather (13)
|
PNSVFY |
30.49 |
NS |
- |