| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ongley, Lauren (10)
|
TYST |
44.61 |
42.06 |
-2.55 |
| 2 |
Harrison, Lucy (9)
|
WRN |
44.27 |
42.52 |
-1.75 |
| 3 |
Gracey-Dalton, Karsyn A (10)
|
NIBRAD |
43.35 |
42.72 |
-0.63 |
| 4 |
Crosscut, Madison N (10)
|
COUN |
47.84 |
44.05 |
-3.79 |
| 5 |
Cage, Jaslene (8)
|
ZZESYS |
45.68 |
44.66 |
-1.02 |
| 6 |
Keebler, Danielle (10)
|
OCY |
45.50 |
44.74 |
-0.76 |
| 7 |
Kissell, Madyson (9)
|
OCY |
46.87 |
44.86 |
-2.01 |
| 8 |
Crocker, Jenna (10)
|
TYST |
45.99 |
45.46 |
-0.53 |
| 9 |
Goodwill, Mahra (9)
|
COR |
45.97 |
45.83 |
-0.14 |
| 10 |
Colwell, Liz (9)
|
COR |
49.35 |
48.33 |
-1.02 |
| 11 |
Mong, Kallie (10)
|
OCY |
49.35 |
48.55 |
-0.80 |
| 12 |
Sontheimer, Avery (10)
|
COR |
50.97 |
49.15 |
-1.82 |
| 13 |
Maloney, Ava (9)
|
ZZESYS |
47.98 |
50.11 |
2.13 |
| 14 |
Stutts, Makayla (9)
|
COUN |
51.20 |
50.57 |
-0.63 |
| 15 |
Myers, Mercedes (10)
|
FLY |
51.48 |
50.61 |
-0.87 |
| 16 |
Collier, Lillian (10)
|
NIBRAD |
56.72 |
51.35 |
-5.37 |
| 17 |
Abplanalp, Traci (9)
|
WRN |
56.70 |
54.83 |
-1.87 |
| 18 |
Shimmel, Zoey (9)
|
YJET |
1:00.62 |
59.79 |
-0.83 |
| 19 |
Schwabenbauer, Addison (9)
|
COR |
59.94 |
1:00.56 |
0.62 |
| 20 |
Stutzman, Reagan (9)
|
YJET |
1:11.73 |
1:01.98 |
-9.75 |
| 21 |
Williams, Isabella L (10)
|
COUN |
1:04.25 |
1:02.66 |
-1.59 |
| 22 |
Fisher, Ella (10)
|
OCY |
1:09.21 |
1:05.41 |
-3.80 |
| 23 |
Watkins, Elora (10)
|
YJET |
2:02.69 |
1:50.40 |
-12.29 |
|
Weinlein, Millicent (9)
|
OCY |
1:37.67 |
DQ |
- |
|
Stevenson, Lilly (9)
|
TYST |
59.38 |
DQ |
- |
|
Caudill, Brady (9)
|
WRN |
1:02.41 |
DQ |
- |