| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Albright, Hallie (10)
|
WICVY |
30.49 |
28.58 |
-1.91 |
| 2 |
Skutley, Anna E (10)
|
WIECY |
31.89 |
32.60 |
0.71 |
| 3 |
Berg, Piper (9)
|
WICVY |
35.25 |
36.29 |
1.04 |
| 4 |
Zwiefelhofer, Isabella (9)
|
WICVY |
36.05 |
36.51 |
0.46 |
| 5 |
Boos, Gracie (10)
|
WICVY |
36.32 |
36.99 |
0.67 |
| 6 |
Goldbach, Emma R (10)
|
WIECY |
38.96 |
37.37 |
-1.59 |
| 7 |
Wheeler, Claraissa (10)
|
WICVY |
40.53 |
37.94 |
-2.59 |
| 8 |
Slowiak, Addison (9)
|
WICVY |
41.25 |
39.39 |
-1.86 |
| 9 |
Chumas, Athena F (9)
|
WIECY |
41.51 |
40.27 |
-1.24 |
| 10 |
Burcaw, Ann Marie R (10)
|
WIECY |
42.80 |
40.96 |
-1.84 |
| 11 |
Freehling, Grace (10)
|
WICVY |
44.75 |
44.97 |
0.22 |
| 12 |
Kidd, Claire (9)
|
WICVY |
45.91 |
45.00 |
-0.91 |
| 13 |
Schuelke, Lorelai (9)
|
WICVY |
46.38 |
48.43 |
2.05 |
| 14 |
Jacob, Anoushka R (9)
|
WIECY |
|
48.64 |
- |
| 15 |
McCormick, Evangeline G (9)
|
WIECY |
|
52.65 |
- |
| 16 |
Zeug, Madeline (10)
|
WICVY |
1:13.79 |
56.92 |
-16.87 |
| 17 |
Meinen, Leah (9)
|
WICVY |
1:08.23 |
58.46 |
-9.77 |