| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fuse, Lacey M (16)
|
WIECY |
25.54 |
25.79 |
0.25 |
| 2 |
Heinz, Ava (14)
|
WICVY |
27.48 |
27.27 |
-0.21 |
| 3 |
Johnson, Camille S (13)
|
WILAXY |
27.04 |
27.31 |
0.27 |
| 4 |
Archibald-Smith, Paige G (14)
|
WIECY |
27.69 |
27.35 |
-0.34 |
| 5 |
Vierbicher, Nicole A (16)
|
WIECY |
26.29 |
27.42 |
1.13 |
| 6 |
Beam, Jenna A (13)
|
WILAXY |
27.24 |
27.93 |
0.69 |
| 7 |
Beranek, Jenna (13)
|
WICVY |
29.06 |
28.57 |
-0.49 |
| 8 |
Stary, Charlie (14)
|
WICVY |
29.45 |
28.90 |
-0.55 |
| 9 |
Beltz, Natalie E (15)
|
WIECY |
29.94 |
30.17 |
0.23 |
| 10 |
Miller, Cara A (14)
|
WIECY |
28.79 |
30.23 |
1.44 |
| 11 |
Zarecki, Noelle (14)
|
WILAXY |
29.91 |
30.28 |
0.37 |
| 12 |
Schuelke, Emma (13)
|
WICVY |
34.11 |
32.71 |
-1.40 |
| 13 |
Siegert, Melanie (14)
|
WICVY |
|
33.12 |
- |
| 14 |
Hardt, Adia (13)
|
WICVY |
34.56 |
35.59 |
1.03 |
| 15 |
Wundrow, Joscelin (14)
|
WICVY |
36.04 |
36.51 |
0.47 |