| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shao, Emma X (9)
|
ADUN |
|
46.67 |
- |
| 2 |
Kane, Emily J (10)
|
ADCLRK |
|
51.11 |
- |
| 3 |
Deal, Grace A (10)
|
ADCLIN |
1:03.26 |
52.10 |
-11.16 |
| 4 |
Donofrio, Sophia B (10)
|
ADCPP |
52.37 |
53.07 |
0.70 |
| 5 |
Allinson, Mae L (9)
|
ADDELM |
1:00.08 |
54.59 |
-5.49 |
| 6 |
Zhang, Annabelle Y (9)
|
ADCPP |
55.48 |
55.36 |
-0.12 |
| 7 |
Mieu, Shayla S (10)
|
ADBFIN |
53.47 |
55.67 |
2.20 |
| 8 |
Haberbusch, Shelby A (10)
|
ADCLIN |
|
55.79 |
- |
| 9 |
Relyea, Cadence G (8)
|
ADDELM |
50.92 |
56.01 |
5.09 |
| 10 |
Cho, Anna Y (8)
|
ADALBS |
|
59.02 |
- |
| 11 |
Stoup, Madelyn L (9)
|
ADCPP |
58.53 |
1:01.43 |
2.90 |
| 12 |
De Tuya, Elizabeth M (10)
|
ADDELM |
57.47 |
1:01.90 |
4.43 |
| 13 |
Wultsch-Fuller, Sonja E (9)
|
ADNTS |
1:03.35 |
1:03.38 |
0.03 |
| 14 |
Pelnik-Wadsworth, Kaitryn M (10)
|
ADCLIN |
1:07.12 |
1:05.19 |
-1.93 |
| 15 |
O'Donnell, Bridgett M (9)
|
ADEELS |
1:14.34 |
1:05.29 |
-9.05 |
| 16 |
Ilardi, Taylor E (10)
|
ADALBS |
1:03.52 |
1:05.87 |
2.35 |
| 17 |
Hopke, Lillian G (7)
|
ADCPP |
1:06.27 |
1:06.06 |
-0.21 |
| 18 |
Patel, Arya S (10)
|
ADCLRK |
1:07.94 |
1:08.12 |
0.18 |
| 19 |
Ramani, Akshaya K (8)
|
ADCPP |
|
1:08.84 |
- |
| 20 |
Krenav, Anna E (8)
|
ADEELS |
|
1:09.09 |
- |
| 21 |
Robles, Isabella M (10)
|
ADDELM |
56.08 |
1:09.75 |
13.67 |
| 22 |
Harpootlian, Leah C (8)
|
ADCPP |
1:18.21 |
1:11.22 |
-6.99 |
| 23 |
Zhou, Yixuan (8)
|
ADCPP |
|
1:13.50 |
- |
| 24 |
Lim, Yi Wern (7)
|
ADCPP |
|
1:15.30 |
- |
| 25 |
Cohen, Evyn B (10)
|
ADCLIN |
1:24.94 |
1:15.38 |
-9.56 |
| 26 |
Phillips, Leah M (10)
|
ADCLRK |
1:18.41 |
1:16.53 |
-1.88 |
| 27 |
Miron, Sofia L (9)
|
ADBFIN |
|
1:23.01 |
- |
| 28 |
Dainotto, Olivia F (10)
|
ADCLIN |
1:23.86 |
1:24.04 |
0.18 |
|
Miller, Isabella M (8)
|
ADCPP |
1:11.14 |
DQ |
- |
|
White, Nicole E (9)
|
ADNTS |
1:29.46 |
DQ |
- |