| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baker, Owen M (10)
|
OHLTPY |
33.68 |
32.76 |
-0.92 |
| 2 |
Lickfelt, Jacob K (10)
|
OHLTPY |
40.24 |
40.74 |
0.50 |
| 3 |
Rader, James G (9)
|
OHHURY |
45.37 |
42.11 |
-3.26 |
| 4 |
Khiliutsich, Aliaksei (9)
|
OHHURY |
44.57 |
44.30 |
-0.27 |
| 5 |
Yee, Dylan C (10)
|
OHLTPY |
43.99 |
45.21 |
1.22 |
| 6 |
Melican, Marc T (9)
|
OHLTPY |
46.73 |
46.37 |
-0.36 |
| 7 |
Srivathsan, Anvith * (10)
|
OHLTPY |
47.60 |
49.33 |
1.73 |
| 8 |
Singer, Andrew J (10)
|
OHLTPY |
51.71 |
51.20 |
-0.51 |
| 9 |
Edamadaka, Arnav S (9)
|
OHLTPY |
54.15 |
51.63 |
-2.52 |
| 10 |
Davis, Ziloh A (9)
|
OHHURY |
1:09.12 |
1:01.79 |
-7.33 |